Andrology Center, Peking University First Hospital, Beijing, China
Purpose: To investigate whether adipose-derived stem cells
(ADSCs) are capable of improving the urinary voiding function
and restoring pathologic changes in a parturition-Induced
stress incontinent rat model and to clarify the mechanism, by which ADSCs exert their beneficial effects by VEGF paracrine.
Materials and Methods: Rats were induced to develop stress
urinary incontinence (SUI) by postpartum vaginal balloon
dilation and bilateral ovariectomy. ADSCs were isolated from
the periovary fat and labeled with thymidine analog 5-ethynyl-
2-deoxyuridine (EdU). Twenty rats received urethral injection
of phosphate-buffered saline (PBS). Twenty rats received
urethral injection of EdU-labeled ADSCs. Four weeks later,
the urinary voiding function was assessed by cystometry.
Histological examination (Masson's trichrome stain, picrosirius
red stain, Hart's elastin stain, Gordon & Sweet's stain and
immunohistochemical stain) and western blot were performed.
Results: Cystometric analysis showed that ADSCs treatment
resulted in significant recovery of urinary voiding function, as
compared with SUI group. Histological analysis showed that
ADSCs are capable of restoring the fibrous-muscular system in
the urethra. There was more periurethral blood vessels in the
ADSCs treated group compared with SUI group. The expressions
of VEGF and P-ERK1/2 protein were higher in ADSCs treated
Conclusions: ADSCs are capable of restoring the urethra
fibrous-muscular system. VEGF paracrine and activation of
ERK1/2 by ADSCs might play an important role in restoring
pathologic changes of SUI.