MR 17. Azoospermia factor microdeletion and male infertility

Objective: To explore the relationship between idiopathic azoospermia and severe oligozoospermia with microdeletions of azoospermia factor (AZF) on Y chromosome in male.

Methods: With adoption of the Multiplex PCR (pol-ymerase chain reaction) to detect the 9 sequence-tagged sites (STS) in AZF region of both the 200 currently married men with normal fertility of the control group and the 804 male infertility clients of the experimental group.

Rusults: No microdeletions of AZF was found in the control group, and 90 patients were detected in the experimental group. The total deletion rate was 11.19% (90/804), and it was 11.99% (70/584) for azoospermia and 9.09% (20/220) for severe oligozoospermia respectively. The deletion rate of the azoospermia on Y chromosome in male was obviously higher than that of the severe oligozoospermia, there was significant statistical difference between them (P<0.05). The rate of detection of the 9 sequence-tagged sites (STS) was raised to 2.3% (2/88) than the use of the 6 STS recommended by European Academy ofAndrology (EAA).

Conclusions: The microdeletions of AZF is one of the impor tant reasons of idiopathic azoospermia, severe oligozoospermia and oligozoospermia. Increasing the sequencetagged sites of detection is beneficial to raise the detection rate of microdeletions of AZF.