AB222. Enolase1 (ENO1) and glucose-6-phosphate isomerase (GPI) are good markers to predict human sperm freezability
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AB222. Enolase1 (ENO1) and glucose-6-phosphate isomerase (GPI) are good markers to predict human sperm freezability

Xuping Jiang, Shangqian Wang, Wei Wang, Yang Xu, Hongyong Sun, Zengjun Wang, Wei Zhang

Department of Urology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China


Objective: Sperm cryopreservation is a method to preserve sperm samples for a long period. However, the fertility of sperm decreases markedly after freezing and thawing in a certain amount of samples. The aim of the present study was to find useful and reliable predictive biomarkers of the capacity to withstand the freeze-thawing process in human ejaculates.

Methods: We chose the two proteins as probable markers of sperm freezing capacity. Ejaculate samples were separated into good freezability ejaculates (GFE) and poor freezability ejaculates (PFE) according to progressive motility of the sperm after thawing. Before starting cryopreservation protocols, the two proteins from each group were compared using western blot analysis and immunofluorescence.

Results: Results showed that normalized content of enolase1 (ENO1) (P<0.05) and glucose-6-phosphate isomerase (GPI) (P<0.01) were both significantly higher in GFE than in PFE. The association of ENO1 and GPI with post thaw sperm viability and motility was confirmed using Pearson’s linear correlation.

Conclusions: In conclusion, ENO1 and GPI can be used as markers of human sperm freezability before starting the cryopreservation procedure.

Keywords: Cryopreservation; sperm; enolase1 (ENO1); glucose-6-phosphate isomerase (GPI)


doi: 10.21037/tau.2016.s222


Cite this abstract as: Jiang X, Wang S, Wang W, Xu Y, Sun H, Wang Z, Zhang W. Enolase1 (ENO1) and glucose-6-phosphate isomerase (GPI) are good markers to predict human sperm freezability. Transl Androl Urol 2016;5(Suppl 1):AB222. doi: 10.21037/tau.2016.s222

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