AB79. Establishment of the high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T>C
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AB79. Establishment of the high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T>C

Hongen Lei, Zhezhu Gao, Yongde Xu, Zhongcheng Xin, Yinglu Guo, Ruili Guan

Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China

Objective: To establish a high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T>C.

Methods: Two unlabeled probes were designed. One is complementary to wild type and another is complementary to the mutant type. 71 health people were performed by HRM with unlabeled probes for genotyping CCL2-2518 T>C, and TaqMan method was used to verify the results from above.

Results: The results using the HRM with unlabeled probes were consistent with that using TaqMan method. The difference between the temperature in two probe peaks by using the probe complementary to wild type is 2.1°C; and the difference between the temperature in two probe peaks by using the probe complementary to mutant type is 5.5°C.

Conclusions: Establishment the high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T>C is a simple, economic, sensitive, accurate, and fast technique, which could be used in clinical inspection.

Keywords: high resolution melting analysis; genotyping CCL2-2518 T>C; clinical inspection

doi: 10.3978/j.issn.2223-4683.2014.s079

Cite this article as: Lei H, Gao Z, Xu Y, Xin Z, Guo Y, Guan R. Establishment of the high resolution melting analysis with unlabeled probes for genotyping CCL2-2518 T>C. Transl Androl Urol 2014;3(S1):AB79. doi: 10.3978/j.issn.2223-4683.2014.s079

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