MR 29. SEMG1 may be the candidate gene for idiopathic asthenozoospermia

Objective: To illustrate the biological mechanisms and in search of candidate gene for asthenozoosperm.

Methods: In this study, we examined the gene expression profiles of asthenozoosperm. Gene expression profiles analyses with microarray on spermatozoa specimens from 12 asthenozoosperm patients and 12 age-match volunteers were performed, data analysis was performed with bioinformatics tools.

Results: Data analysis revealed that 1,265 and 262 genes were significantly (P<0.05) and differently expressed (≥2-fold) between groups performed with Genespring and BRB-Array Tools, respectively. Of these differently expressed genes, seventy-one were identified as molecular signatures of asthenozoosperm, of which most involved in primary metabolic process and cellular metabolic process. Molecular signatures were filtered performed with Nextbio, twenty-one genes were indentify to be specially expressed in asthenozoosperm. We used FACTA to match the specially expressed genes against the MEDLINE database and found SEMG1, PGAP1 were related to male-fertility. Validation of the microarray data of SEMG1 was carried out using real-time PCR.

Conclusions: SEMG1 remarkably changes in asthenozoosperm, and therefore may be the candidate gene for the development of diagnostic marker and provides the opportunity to further illustrated the biological mechanisms of asthenozoosperm.