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Microenergy acoustic pulses induced myogenesis of urethral striated muscle stem/progenitor cells

	author = {Kai Cui and Ning Kang and Lia Banie and Tie Zhou and Tianshu Liu and Bohan Wang and Yajun Ruan and Dongyi Peng and Hsun Shuan Wang and Tianyu Wang and Guifang Wang and Amanda B. Reed-Maldonado and Zhong Chen and Guiting Lin and Tom F. Lue},
	title = {Microenergy acoustic pulses induced myogenesis of urethral striated muscle stem/progenitor cells},
	journal = {Translational Andrology and Urology},
	volume = {8},
	number = {5},
	year = {2019},
	keywords = {},
	abstract = {Background: Stress urinary incontinence (SUI) is a common disorder with high prevalence in women across their life span, but there are no non-surgical curative options for the condition. Stem cell-based therapy, especially endogenous stem cell therapy may be a potential treatment method for SUI. The aims of this study are to identify, isolate, and assay the function of urethral striated muscle derived stem/progenitor cells (uMDSCs) and to assess uMDSC response to microenergy acoustic pulses (MAP).
Methods: Urethral striated muscle was identified utilizing 3D imaging of solvent organs (3DISCO) and immunofluorescence (IF). uMDSCs were isolated and purified from Zucker Lean (ZL) (ZUC-LEAN) (ZUC-Leprfa 186) rats, with magnetic-activated cell sorting (MACS) and pre-plating methods. The stemness and differentiation potential of the uMDSCs were measured by cell proliferation, EdU, flow cytometry, IF, and Western blot.
Results: Comparison of the cell proliferation assays between MACS and pre-plating reveals the advantage of MACS over pre-plating. In addition, the study reveals that uMDSCs form myotubes when treated with MAP.
Conclusions: The uMDSCs within female rat urethral striated muscle could be a therapeutic target of MAP in managing SUI.},
	issn = {2223-4691},	url = {}