AB118. Effect of MOTILIPERM in cisplatin administered SD rats
Poster Presentation

AB118. Effect of MOTILIPERM in cisplatin administered SD rats

Kiran Kumar Soni1, Li Tao Zhang1, Bo Ram Choi1, Yu Seob Shin1, Jong Kwan Park1, Sung Won Lee2, Chul Young Kim3, Wan-Shou Cui4, Han Jung Chae5, Hye Kyung Kim6

1Department of Urology, Chonbuk National University Medical School and Institute for Medical Sciences, Chonbuk National University and Biomedical Research Institute and Clinical Trial Center for Medical Devices of Chonbuk National University Hospital, Jeonju, Republic of Korea; 2Department of Urology, Sungkyunkwan University Medical School, Seoul, Republic of Korea; 3College of Pharmacy, Hangyang University, Ansan 426-791, Republic of Korea; 4Andrology Center, Peking University First Hospital, Beijing 100034, China; 5Department of Pharmacology, Chonbuk University Medical School, Jeonju, Republic of Korea; 6College of Pharmacy, Kyungsung University, Busan 48434, Republic of Korea


Objective: Cisplatin causes male infertility but the exact mechanism has not been clarified, yet. MOTILIPERM has been implicated in alleviation of infertility in Sprague-Dawley rats caused by cisplatin. We evaluated recovery effect of MOTILIPERM on cisplatin (CIS)-induced testicular toxicity in Sprague-Dawley rats.

Methods: Five groups were included. The groups are control (CTR), CTR + MOTILIPERM 200 mg/kg/day per oral, CIS 10 mg/kg i.v., CIS 10 mg/kg + MOTILIPERM 100 mg/kg/day, CIS 10 mg/kg + MOTILIPERM 200 mg/kg/day. CIS 10 mg/kg i.v. single dose was given before 100 or 200 mg/kg MOTILIPERM per oral daily for 28 days. Body and genital organs weight, epididymis sperm count, sperm motility, sperm apoptosis, testosterone level, MDA of testis tissue, spermatogenic cell density, and Johnsen’s score were evaluated. Steroidogenic acute regulatory (StAR) protein, and Glucose-regulated protein-78 (GRP-78), phosphorylated inositol-requiring transmembrane kinase/endoribonuclease 1 (ire1) and phosphorylated c-jun-N-terminal kinase (p-JNK) were quantitated by western blot to show its signaling pathway.

Results: The body weight was decreased significantly in CIS 10 mg/kg, CIS 10 mg/kg + MOTILIPERM 100 mg/kg/day, CIS 10 mg/kg + MOTILIPERM 200 mg/kg/day compared with CTR (P<0.001) however, it was increased in CIS 10 mg/kg + MOTILIPERM 100 mg/kg/day, CIS 10 mg/kg + MOTILIPERM 200 mg/kg/day compared with CIS 10 mg/kg. The decreased weight of epididymis and prostate were increased significantly in CIS 10 mg/kg + MOTILIPERM 100 mg/kg/day compared with CIS 10 mg/kg. Sperm count, sperm motility, sperm apoptosis, MDA of testis tissue, spermatogenic cell density, Johnsen’s score, and total testosterone were also significantly improved by MOTILIPERM treatment. The levels of decreased StAR protein were significantly improved by MOTILIPERM administration, increased GRP-78 protein p-IRE1and p-JNK was also significantly decreased with MOTILIPREM treatment.

Conclusions: The MOTILIPERM could be an effective medicine to reduce the toxic effect caused ER stress by CIS in the testis.

Keywords: Cispaltin (CIS); MOTILIPERM; spermatogenic cell density; steroidogenic acute regulatory (StAR) protein; glucose-regulated protein-78 (GRP-78); phosphorylated inositol-requiring transmembrane kinase/endoribonuclease 1 (IRE1); phosphorylated c-jun-N-terminal kinase (p-JNK)


doi: 10.21037/tau.2016.s118


Cite this abstract as: Soni KK, Zhang LT, Choi BR, Shin YS, Park JK, Lee SW, Kim CY, Cui WS, Chae HJ, Kim HK. Effect of MOTILIPERM in cisplatin administered SD rats. Transl Androl Urol 2016;5(Suppl 1):AB118. doi: 10.21037/tau.2016.s118

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