AB114. Myogenic differentiation of rat bone mesenchymal stem cells in vitro
Poster Presentation

AB114. Myogenic differentiation of rat bone mesenchymal stem cells in vitro

Heng-Jun Xiao1, Wei-Xin Yan1, Jun Chen1, Xin Gao1, Li Zhuan2, Guang-Hui Zang3, Jun Yang3, Yan Zhang3, Ji-Hong Liu3

1The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China; 2The First People’s Hospital of Yunnan Province, Kunming 650032, China; 3Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China


Objective: To culture and myogenic differentiation of rat bone mesenchymal stem cells (BMSCs) in vitro, and provide the available seed cells for erectile dysfunction (ED) therapy.

Methods: Rat BMSCs were isolated and cultured from the femur and tibia of Sprague Dawley (SD) rat. Mesenchymal stem cell positive cellular markers CD49d, CD73, CD90, CD105, CD106 and negative cellular markers CD31, CD34 and CD45 were arrayed by flow cytometry analysis. Furthermore, the fourth passage cells were induced and identified by their capacities in the myogenic differentiation.

Results: The cultured cells expressed mesenchymal stem cell positive cellular markers CD49d, CD73, CD90, CD105 and CD106, and lacked negative cellular markers CD31, CD34 and CD45. Myogenic differentiation cells can be stained with alpha-smooth muscle actin (α-SMA) and desmin, respectively.

Conclusions: Rat BMSCs have been successfully isolated, cultured and myogenic differentiation in vitro. They could be used as autogenous BMSCs and gene modified BMSCs for ED therapy.

Keywords: Rat; bone mesenchymal stem cells (BMSCs); erectile dysfunction


doi: 10.21037/tau.2016.s114


Cite this abstract as: Xiao HJ, Yan WX, Chen J, Gao X, Zhuan L, Zang GH, Yang J, Zhang Y, Liu JH. Myogenic differentiation of rat bone mesenchymal stem cells in vitro. Transl Androl Urol 2016;5(Suppl 1):AB114. doi: 10.21037/tau.2016.s114

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