AB068. The splicing factor SRSF1 expression in human bladder carcinoma cells

Objective: SRSF1 is a splicing factor often times described as an oncoprotein. In the present work, we examined the expression of SRSF1 in human bladder carcinoma cells and detected the change of SRSF1 expression in BIU-87 cells after the treatment of chemotherapeutic drug.

Methods: Human bladder carcinoma cells (BIU-87) were treated by growth factors with or without chemotherapeutic drug (pirarubicin) agents’ interference. The confirmed of SRSF1 expression in BIU-87 cells and the assessment of the chemotherapeutic drug’s function to SRSF1 protein was analyzed by Western blot.

Results: SRSF1 precisely expressed in human bladder carcinoma cells—BIU-87 cell line. Protein levels of SRSF1 significantly decreased after pirarubicin treatment in a higher concentration (1.6, 3.2 ug/mL) while slightly increased after the treatment in a lower concentration (0.4, 0.8 ug/mL). SRSF1 expression appeared as a peak form.

Conclusions: This study tells the expression of SRSF1 BIU-87 cells and its variation after chemotherapeutic drug (pirarubicin) treatment. In conclusion, SRSF1 may act as a useful potential target for bladder carcinoma therapies.

Keywords: SRSF1; bladder carcinoma; splicing factor; pirarubicin