AB225. The tumor suppressive role of CAMK2N1 in castration-resistant prostate cancer
Abstract publication Basic research

AB225. The tumor suppressive role of CAMK2N1 in castration-resistant prostate cancer

T Wang, Z Liu, S Guo, L Wu, M Li, J Yang, R Chen, H Xu, S Cai, H Chen, W Li, L Wang, Z Hu, Q Zhuang, S Xu, L Wang, J Liu, Z Ye, JY Ji, C Wang, K Chen

Department of Urology, Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, China

Abstract: Prostate cancer at advanced stages including metastatic and castration-resistant cancer remains incurable due to the lack of effective therapies. The CAMK2N1 gene, cloned and characterized as an inhibitor of CaMKII (calcium/calmodulin-dependent protein kinase II), has been shown to affect tumor genesis and tumor growth. However, it is still unknown whether CAMK2N1 plays a role in prostate cancer development. We first examined the protein and mRNA levels of CAMK2N1 and observed a significant decrease in human prostate cancers comparing to normal prostate tissues. Re-expression of CAMK2N1 in prostate cancer cells reduced cellular proliferation, arrested cells in G0/G1 phases, and induced apoptotic cell death accompanied by down-regulation of IGF-1, ErbB2, and VEGF downstream kinases PI3K/AKT, as well as the MEK/ERK-mediated signaling pathways. Conversely, knockdown of CAMK2N1 had a significant opposite effects on these phenotypes. Our analyses suggest that CAMK2N1 plays a tumor suppressive role in prostate cancer cells. Reduced CAMK2N1 expression correlates to human prostate cancer progression and predicts poor clinical outcome, indicating that CAMK2N1 may serve as a biomarker. The inhibition of tumor growth by expressing CAMK2N1 established a role of CAMK2N1as a therapeutic target.

Keywords: Prostate cancer; CAMK2N1 gene; tumor growth

doi: 10.3978/j.issn.2223-4683.2014.s225

Cite this article as: Wang T, Liu Z, Guo S, Wu L, Li M, Yang J, Chen R, Xu H, Cai S, Chen H, Li W, Wang L, Hu Z, Zhuang Q, Xu S, Wang L, Liu J, Ye Z, Ji JY, Wang C, Chen K. The tumor suppressive role of CAMK2N1 in castration-resistant prostate cancer. Transl Androl Urol 2014;3(S1):AB225. doi: 10.3978/j.issn.2223-4683.2014.s225

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