RU 02. Bone marrow mesenchymal stem cells combined with polylactic glycolic acid scaffold for rat neural injury

Objective: To study the effect of bone marrow mesenchymal stem cells (BMSCs) and differentiated neural-like cells combined with polylactic glycolic acid (PLGA) scaffolds on rat nerve injury.

Methods: BMSCs were cultured by differential adherence and composited PLGA. Scanning electron microscope was applied to investigate the information of BMSCs' adhesion, proliferation, and induction into neural-like cells in the scaffolds. Bold scaffold, BMSCs-PLGA complex, and neural-like cells-PLGA complex were separately transplanted into SD rat models with injured right sciatic nerve. The SD rat models were observed within 60 days after operation.

Results: BMSCs and neural-like cells adhered to and proliferated on the PLGA. BMSCs and neural-like cells combined with PLGA restored the nerve injury.

Conclusions: BMSCs and differentiated neural-like cells combined with PLGA scaffolds can be a useful method of tissue engineering for treating neurogenic bladder caused by spinal cord injury and advanced diabetes.