1Department of Urology, First Hospital of Jilin University, Changchun
130021, Jilin, China; 2Department of Urology, Jilin Province People's
Hospital, Changchun 130021, Jilin, China; 3Graduate School of Jilin
University, Changchun 130012, Jilin, China; 4College of Life Science,
Jilin University, Changchun 130012, Jilin, China
Objective: The expression of hMOF protein in renal cell
carcinoma tissues is measured to investigate its association with
the clinical characteristics of renal cancer patients.
Methods: Immunohistochemistry and qRT-PCR were employed
to detect the difference in hMOF expression levels between
normal kidney and renal cell carcinoma tissues and determine
the association between HMOF expression and the clinical
characteristics of renal cell carcinoma patients.
Results: QRT-PCR findings showed that four of the nine
patients (44.4%) had significantly declined mRNA levels.
Immunohistochemical results showed that hMOF protein
expression was negative or weakly positive in the renal cell
carcinoma tissues of 39 patients (62.1%, 39/66), compared
to that in the adjacent normal tissue of only 4 patients (6.1%,
4/66), and the difference was significant (P<0.05). Compared
to Fuhrman I to II, hMOF expression in renal cell carcinoma
Fuhrman Ⅲ was remarkably lower with significant differences
(P<0.05). HMOF expression was significantly lower in poorly
differentiated renal cell carcinoma compared with the welldifferentiated
group, and the differences were also significant
(P<0.05). In addition, patients with renal cell carcinoma and
decreased hMOF protein levels were at risk of metastases to
regional lymph nodes and distant organs.
Conclusions: The expression of hMOF protein is significantly
lower in renal cell carcinoma, particularly those with worse
pathological stage and Fuhrman grade, suggesting an association
between the protein and the occurrence and development of
renal cell carcinoma as well as the severity.