Objective: To investigate the acute damage degree on the
morphology of Leydig cells and testosterone synthesis function
different doses of 60Co-γin rats by.
Methods: Sixty male SD rats were equally randomized into five
groups: Group A: also the normal control group, with 60Co-γ
exposure adopted only over testis; Group B: 4+4 Gy; group C:
6+6 Gy; group D: doses was 2+10 Gy; and group E: 6+8 Gy.The
exposure duration was 24 h. Serum luteinizing hormone (LH)
and testosterone (T) levels were determined by enzyme-linked
immunoassay 48h after of the exposure. The histology of the
testis tissues was observed under microscope. The mRNA levels
in the steroidogenic acute regulatory protein (StAR) and in the
cytochrome P450 side chain cleavage (P450scc) from the Leydig
cells were determined by RT-PCR.
Results: Compared with the normal control (group A), the
levels of serum LH in the groups of 60Co-γ exposure (groups B,
C, D, and E) were significantly higher (P<0.05), and the levels of
serum T were significantly lower than normal (P<0.05). Under
the microscope, the Leydig cells were smaller and less than
normal, cytoplasms were less, cell nucleuses were shrunken, and
chromatins were enriched and anachromasis. The expressions of
mRNA in StAR and P450scc were also significantly lower than
the control group (P<0.05).
Conclusions: The impairment on the morphology of Leydig
cells and testosterone synthesis function is obviously after 60Co-
γ exposure. The damage degree is associated with both the total
doses and the biggest exposure doses.