1Department of Urology, Nanfang Hospital, Guangzhou 510515,
Guangdong Province, China; 2The Third Affiliated Hospital, Southern
Medical University, Guangzhou 510515, Guangdong Province, China;
3Institute of Genetic Engineering, Southern Medical University,
Guangzhou 510515, Guangdong Province, China
Objective: To illustrate the biological mechanisms and in search
of candidate gene for asthenozoosperm.
Methods: In this study, we examined the gene expression profiles
of asthenozoosperm. Gene expression profiles analyses with
microarray on spermatozoa specimens from 12 asthenozoosperm
patients and 12 age-match volunteers were performed, data
analysis was performed with bioinformatics tools.
Results: Data analysis revealed that 1,265 and 262 genes were
significantly (P<0.05) and differently expressed (≥2-fold) between
groups performed with Genespring and BRB-Array Tools,
respectively. Of these differently expressed genes, seventy-one were
identified as molecular signatures of asthenozoosperm, of which
most involved in primary metabolic process and cellular metabolic
process. Molecular signatures were filtered performed with Nextbio,
twenty-one genes were indentify to be specially expressed in
asthenozoosperm. We used FACTA to match the specially expressed
genes against the MEDLINE database and found SEMG1, PGAP1
were related to male-fertility. Validation of the microarray data of
SEMG1 was carried out using real-time PCR.
Conclusions: SEMG1 remarkably changes in asthenozoosperm,
and therefore may be the candidate gene for the development
of diagnostic marker and provides the opportunity to further
illustrated the biological mechanisms of asthenozoosperm.