Aims: Anti-follicle-stimulating hormone (FSH) autoantiboby
was found to highly correlate with oligospermia and
asthenospermia, but the actual effect of FSH autoantibody on
spermatogenesis is still unknown.
Main Methods: In this study, 21-day rats were immunized
seven times with FSH peptides linked with Keyhole Limpet
Hemocyanin (KLH) (experimental group) or KLH (control
group) every two weeks. Luteinizing hormone (LH) and inhibin
B level in the immunized rat sera were measured by enzyme-linked
immunosorbent assay (ELISA). Apoptosis of spermatogenic cells
in the testis was detected by in situ end labeling method (TUNEL),
and the mRNAs of Bax, Bcl-2 and Caspase-3 in testis were detected
by fluorescent Quantitative PCR.
Key findings: Compared with the control, serum inhibin B level
was significantly decreased at all time points (34.49%, 23.20%,
and 37.00%) (P<0.05). There was no difference in the serum LH
level between experimental and control groups. FSH peptide
immunization increased the apoptosis of spermatogenic cells in
the testis that was associated with an imbalance of Bax and Bcl-2
expression and upregulation of Caspase-3.
Significance: these results suggest that FSH autoantibody
could cause the reduction of inhibin B, thereby inducing
hypospermatogenesis via augment of spermatogenic cell