MR 13. The value of epididymal protease inhibitor in differential diagnosis between obstructive azoospermia and non - obstructive azoospermia
Male Reproduction

MR 07. The effect of annexin 5 on the expression of ECT2 involved in the process of cell proliferation from rat Leydig Cells

B. Liu1, S. Su1, P. Wang1, X. Niu1, X. Yang2, W. Zhang1, Z. Wang1, X. Wang3

1Laboratory of Reproductive Medicine, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China; 2Center of Clinical Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China; 3Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing 210029, China

Summary: There are no efficient and noninvasive clinical tests to distinguish between obstructive azoospermia (OA) and non-obstructive azoospermia (NOA). Epididymal protease inhibitor (Eppin) protein is secreted specifically by testes and epididymides in male reproductive system. It does not exist in seminal plasma of patients with OA in theory. The seminal plasma from 40 normal men and 46 azoospermic patients was detected via Western blot for investigating the presence and characteristics of Eppin protein to distinguish between OA and NOA. The cases were diagnosed as NOA whether Eppin in seminal plasma was positive via Western blot analysis. The cases were diagnosed as OA when samples were Eppin-negative. Additionally, percutaneous epididymal sperm aspiration (PESA) and percutaneous testicular sperm aspiration (PTSA) were performed on these patients at the same time as the diagnostic criteria to compare with Western blot analysis. Eppin detection in seminal plasma showed similar effectivity with PESA/PTSA in differential diagnosis between OA and NOA. Compared with PESA/PTSA, Eppin detection is a new, efficient and noninvasive method which has good clinical application.

Key words

Differential diagnosis; epididymal protease; inhibitor; non-obstructive; azoospermia; obstructive azoospermia

DOI: 10.3978/j.issn.2223-4683.2012.s106

Article Options

Download Citation