Objective: To determine if human ejaculated sperm plasma
exist caspases, to analyse the relationship between routine semen
parameter and the levels of Caspase3,8,9 in both sperm and
plasma and to speculate the possible significant.
Methods: Semens were abtained from men consulting for
male infertileity in our hospital and in reproductive center
of Hainan medical school. There were 76 samples in all,
includeing 20 normal samples, 19 asthenospermia samples, 20
oligoasthnotspermia samples and 17 azoospermia samples. All
semen samples were analysis by CASA, and then sperm and
plasma were separated by common centrifugation, at last, a
double-antibody-sandwich-ELISA was used to detect the level of
Caspase3,8,9 in both sperm and plasma.
Results: (I) Caspase3,8,9 were all determined in all examined
samples in both sperms and plasma. (II) When considered as a
whole, the level of Caspase3 and Caspase8 in plasma were both
significantly higher than in sperm (Caspase3: 9.00±9.20 pmol/L vs.
6.36±5.81 pmol/L, P<0.05; Caspase8: 17.32±11.23 pmol/L vs.
12.72±7.42 pmol/L, P<0.05). (III) When considered as a whole,
the levels of Caspase8 and Caspase9 in sperm were respectively
negtively correlated with sperm concentration, motility and
A%; while in plasma, Caspase8 and Caspase9 were respectively
negatively correlated with sperm A%, and only the level of
Caspase8 was negatively correlated with sperm concentration.
However, when considered as each group, only in normal group,
sperm concentration was negatively correlated with Caspase9
in sperm. Of course, all the correlation mentioned above were
significant. (IV) Compared with the normal group, only the
oligoasthnotspermia group exhibited significantly increased level
of Caspase8 in sperm (P<0.05).
Conclusions: (I) Caspases also exist in sperm plasma, which
may reflect the situation of sperm apoptotic in testis and /or
epididymis, to a large extent. (II) Caspase8,9 in sperm can be a
good indicator for poor sperm quality.