1Capital Medical University Affiliated Xuanwu Hospital, Beijing
100048, China; 2China Meitan General Hospital of State
Administration of Work Safety, Beijing, 100028, China; 3Peking
University First Hospital, Beijing 100034, China
Cox7a2 is the gene encoding one component of the nuclearencoded
subunits of cytochrome c oxidase complex in the
mitochondrial respiratory chain. Cox7a2 had been shown high
expressed in the testes of aging males, but the significance of its
high expression pattern was not clear presently. In our study, we
investigated the effect of Cox7a2 on testosterone production in
TM3 mouse Leydig cells, and the involvement mechanism of this
effect. Cox7a2-pEYFP-N1 plamid was construced and transfected
back to TM3 mouse Leydig cells for the overpression of Cox7a2.
The testosterone secretion was tested by ELISA and the expression
of StAR, P450scc and 3β-HSD was investigated by western blotting.
The investigations suggested that Cox7a2 inhibited the LH-induced
testosterone production at least by disrupting the expression of
StAR protein, other than the expression of P450scc and 3β-HSD
enzymes in TM3 mouse Leydig cells.