New case of secondary bladder amyloidosis with massive hematuria—role of intravesical instillations with dimethyl sulfoxide
Case Report

New case of secondary bladder amyloidosis with massive hematuria—role of intravesical instillations with dimethyl sulfoxide

Esther García Rojo1, Daniel A. González Padilla1, Alicia Castelo Loureiro2, Lucía Parrilla Rubio3, Ángel Tejido Sánchez1, Félix Guerrero Ramos1

1Urology Department, 2Oncology Department, 3Pathology Department, University Hospital 12 de Octubre, Madrid, Spain

Correspondence to: Esther García Rojo. Department of Urology, University Hospital 12 de Octubre, Av. Córdoba SN, 29041, Madrid, Spain. Email: esthergrojo@hotmail.com.

Abstract: Secondary bladder amyloidosis is a rare condition with less than 60 cases published in the world. It is usually secondary to chronic inflammatory processes such as rheumatologic diseases. Hematuria is its predominant and most important symptom, and usually occurs after a bladder catheterization. The diagnostic confirmation is made through a pathological and immunohistochemical study. The treatment must be staggered from less to more invasive. Our objectives are to present a new case of secondary bladder amyloidosis in a woman with a history of chronic bronchiectasis after tuberculosis and frequent super infections, whose main manifestation was a massive hematuria, and review this rare pathology. We have obtained very good initial results using intravesical instillations with dimethyl sulfoxide (DMSO) with complete resolution of the hematuria, the patient remaining asymptomatic for 6 months. After that, there was a recurrence of the hematuria that was treated with embolization of the hypogastric arteries, with good results. We can conclude that, despite being a rare condition, we must consider secondary bladder amyloidosis in patients who have already been diagnosed with systemic amyloidosis and/or chronic pathologies who develop hematuria after bladder catheterization. Based on our experience, instillations with dimethyl sulfoxide are a safe option and provide a quick and temporary resolution of hematuria symptoms.

Keywords: Secondary bladder amyloidosis; massive hematuria; dimethyl sulfoxide instillations


Submitted Jun 07, 2019. Accepted for publication Sep 10, 2019.

doi: 10.21037/tau.2019.09.22


Introduction

Amyloidosis (named for its staining similarities with starch) is an entity described by Rokitansky, but it owes its name to Wirchow, who named it this way in the mid-nineteenth century (1). It is a pathology characterized by an extracellular deposit of proteins called amyloidal material that with its progressive deposit, ends up causing an organ or tissue deterioration.

There are two types of amyloidosis based on location, localized and systemic. Furthermore, depending on the type of accumulated fibers, the disease can be classified into AL or primary amyloidosis and AA or secondary amyloidosis. In primary amyloidosis there is no evidence of a pathology responsible for the condition (except multiple myeloma) and it usually includes cardiovascular, gastrointestinal, skin, thyroid and lung involvement. AA or secondary amyloidosis, is related to a chronic inflammatory disease, being rheumatoid arthritis the most frequent cause, although in older papers it was tuberculosis (2) and typically comes with renal, suprarenal glands, lymphatic nodes and spleen affectation.

Other subtypes of amyloidosis are senile amyloidosis, amyloidosis associated with hemodialysis and AF amyloidosis, which is associated with a specific entity called familiar amyloidal polyneuropathy,

Its location in the genitourinary system is variable and can occur anywhere: kidneys, pelvis, ureters, bladder, urethra or even penis (3). The kidney is classically the most affected organ, especially in secondary amyloidosis, although it can also be affected in primary amyloidosis (4). Renal amyloidosis is one of the most frequent causes of nephrotic syndrome (5).

Bladder amyloidosis is a rare entity, being primary bladder amyloidosis more common than secondary (which is a rarity with less than 60 cases published in the world) (6,7). The most preferred site of amyloid deposit is the posterior bladder wall (68%), followed by the trigone (26%) (8). Macroscopic hematuria, with or without clots, is the predominant and frequently the unique clinical manifestation, although it can also manifest as irritative voiding symptoms such as dysuria or pollakiuria (9). Without adequate treatment, the evolution of these patients can be complicated (10) and even fatal (11).

Regarding its endoscopic appearance, it is common to confuse it with an urothelial bladder tumor, sometimes with an infiltrating appearance, especially in cases of primary bladder amyloidosis (12-15). It can also be similar to a glandular cystopathy or inflammatory entities such as cystitis, being more typical in secondary cases (8). This is the reason why a histopathological study of the tissues is mandatory to obtain an accurate diagnosis.

The gold standard for the diagnosis is the histological and immunohistochemical study of the affected tissue, using special staining techniques with haematoxylin and/or Congo Red to demonstrate the presence of amyloid material. The apple green birefringence is typical when observing the tissue in the polarizing microscope with the last-mentioned dye. It is possible to make a differentiation between the type of amyloidosis fibers using potassium permanganate (KMnO4): AL deposits tend to persist, while AAs are usually digested. Other techniques that can be used are immunohistochemical techniques or monoclonal antibodies (16).

Once the disease is confirmed, there is a wide range of therapeutic alternatives depending on the severity of the case: from observation and conservative management with continuous bladder irrigation with saline in mild cases, to more aggressive measures ranging from transurethral resections (TUR) with resection/cauterization of the affected areas, to embolization of the hypogastric arteries or, in severe cases, even to a partial or total cystectomy (8,17).

Other measures that have been used for symptomatic control are intravesical instillations with different substances. One of them that seems to have had promising results is dimethyl sulfoxide (DMSO). DMSO has been shown in vivo to cause degradation of amyloid deposits (18,19). Although its administration regimen is not clear (6,20), it seems that together with a TUR of the lesions, it is the most effective treatment described in the literature in order to eliminate residual amyloid deposits and to achieve symptom control (6,21-23). Most of the treated cases were affected by primary bladder amyloidosis but it seems that this therapy is also effective on secondary amyloidosis deposits (22).

Regarding to subsequent follow-up, it is important to perform periodic cystoscopies to diagnose possible recurrences or possible coexisting malignancies (20,24,25).


Clinical case

We present the case of a 71-year-old woman, born in Peru. Her family and personal history are summarized in Table 1.

Table 1
Table 1 Relevant family and personal history
Full table

The patient arrives to the emergency room with epigastric abdominal pain that worsens with ingestion, accompanied by significant constipation and constitutional syndrome with loss of up to 10 kg of weight during a period of 6 months. No fever, vomiting, hematemesis, rectal bleeding or black stools.

Her relevant emergency blood tests results are, a creatinine of 1.46 mg/dL, potassium of 3.72 mEq/L, a microcytic anemia with 7.5 g of hemoglobin, a C-reactive protein of 19 mg/dL and a hypoalbuminemia of 2.19 g/L.

The subsequent study is summarized in Table 2.

Table 2
Table 2 Diagnostic test and medical procedures
Full table
Figure 1 Colonoscopy images with mucosa of normal appearance.
Figure 2 Thoracic CT images. (A) Bilateral pulmonary bronchiectasis; (B) bulla in right upper lobe.
Figure 3 Abdominal-pelvic CT images. (A) Radiodense content in more distal portion of small intestine; (B) urinary bladder without alterations.

During hospitalization, a bladder catheter is inserted for diuresis quantification, starting a macroscopic hematuria of significant amount, requiring a urological evaluation. The patient does not report previous episodes of hematuria. Conservative measures are taken, such as placement of a catheter for bladder washing with continuous irrigation of saline. In the following days the hematuria with significant anemia persists, requiring transfusion support and a cystoscopy, which is not useful due to the existence of a large organized clot occupying almost the entire bladder. Urgent exploration under anesthesia is decided, performing a cystoscopy with clots evacuation. We find a bladder with a lot of neovascularization, vascular tangles reminiscent of a chronic cystopathy and with multiple venous points that bleed during bladder distension. No exophytic lesions are observed. The bleeding points are coagulated and multiple randomized biopsies are taken (Figure 4).

Figure 4 Bladder endoscopy with biopsy sampling.

After this intervention, the patient continues with severe hematuria, requiring transfusion of a total of 10 packed red blood cells. A new endoscopic surgery with coagulation of the bleeding vessels is performed.

Finally, we obtain the results of both gastrointestinal, bone marrow and bladder mucosa biopsies, all of them with AA amyloid deposits compatible with secondary amyloidosis (Figure 5).

Figure 5 Bladder biopsies (×200). (A) Deposit of perivascular eosinophilic material in hematoxylin/eosin stain; (B) characterization of amyloid type by amyloid-specific amyloid AA immunohistochemical technique; (C) thioflavine histochemical technique, with positive perivascular eosinophilic deposits.

Hematuria persists in the following days. After reviewing the literature, it is decided to start with vesical instillations with dimethyl sulfoxide. We have experience using this substance for the treatment of interstitial cystitis and complex regional pain syndrome. Furthermore, it is easily available and generally well tolerated. We decide to use the same treatment schedule as for the treatment of these diseases, starting with weekly instillations of 50 mL, keeping the substance at least 30 minutes inside the bladder, a pattern that is maintained for 6 weeks and then monthly maintenance instillations, which will be maintained for at least a year. For the instillations, a Foley is inserted just to introduce the substance and then the patient empties the bladder spontaneously.

It is worth highlighting the complete resolution of the hematuria after the first instillation, with the possibility of the catheter removal within 2 days. The patient remains with good voiding quality without irritative symptoms and without recurrence of hematuria after 6 months of follow-up. The instillations were well tolerated and no related complications were observed.

Subsequently, at the 7th month of evolution, the patient developed an episode of macroscopic hematuria that required transfusion of 2 units of packed red blood cells, an endoscopic exploration with evacuation of clots and a selective embolization of the hypogastric arteries to control it. After these measures, the patient progressed favorably, remaining with clear urine and blood analysis with stable hemoglobin level. It was decided to resume the instillations with DMSO, remaining clinically stable and without hematuria for 4 months after the last episode. The total follow-up is 11 months.


Discussion

Amyloidosis is a condition that includes multiple manifestations derived from the abnormal deposition of amyloid protein in tissues. Secondary amyloidosis is reactive to chronic inflammatory processes (2).

In the urinary tract, kidney involvement occurs frequently in secondary amyloidosis (3). In our case, we do not have a renal biopsy, but there are data in favor of renal affectation, such as the existence of renal failure and proteinuria, although not with nephrotic range. In contrast, involvement of the lower urinary tract is uncommon, although its manifestations may be important (26).

The bladder is usually affected in cases of primary amyloidosis (6), being secondary amyloidosis involvement infrequent, with very few cases published in the literature (4,11). Rheumatoid arthritis has been described as the disease most commonly related to these cases, unlike our case, that is secondary to chronic bronchiectasis with frequent superinfections.

The most frequent clinical manifestation of bladder amyloidosis is hematuria, with or without irritative voiding symptoms, and it is often a massive hematuria with even fatal outcomes (11). It is common for a transurethral catheter to act as a trigger for massive hematuria, as in our case (27,28). Its diagnosis is sometimes a real challenge, due to the lack of symptoms specificity and unusual cystoscopic findings (7,29). Its management should be sequential, from less to more aggressive treatments.

The administration of bladder instillations with dimethyl sulfoxide (DMSO) as a local treatment after endoscopic resection, seems to be an effective option for the control of this entity in recurrent cases of both primary and secondary bladder amyloidosis (6,21-23). The actual action mechanism of this substance is unknown, but it has been observed to cause a degradation of the amyloid fibers, without significant side effects (30,31). Internal use, skin application and enema administration have been successfully tried since the efficacy of DMSO for amyloidosis was reported (32). This therapy is usually used orally in cases of systemic amyloidosis or gastric localized amyloidosis (18,30,31,33). A topical application has also been used for cutaneous amyloidosis (34) and due to the high penetrance action of DMSO, which passes with a high concentration rapidly through the skin, some cases of localized amyloidosis of the urinary bladder and ureter, have been effectively treated with occlusive dressing therapy using DMSO in a impregnated gauze (35-37).

The work of Malek et al. exposes the experience of these instillations in 6 patients with primary bladder amyloidosis. They receive instillations every 2 weeks for 3 months, 6 months or a year, depending on the evolution. In two of the patients the therapy failed, requiring in one of them cystectomy (6).

Yoshino et al. (32) treated their case of residual primary bladder amyloidosis after TUR with percutaneous therapy with dimethyl sulfoxide, obtaining also satisfactory results after the treatment, with normalization of cystoscopy and magnetic resonance and without significant side effects.

The work of Diez Calzadilla et al., exposes another case of massive hematuria due to bladder amyloidosis secondary to a recurrent rheumatoid arthritis. After a TUR, selective embolization and instillations with aluminum potassium and DMSO every 72 hours were administered with good control of the hematuria (10). A similar case in a young patient, with the same basal pathology and recurrent hematuria, was treated by the group of Nurmi et al. with a pattern of biweekly instillations for a year (22).

Based on these previous experiences, we have designed a weekly instillation pattern that we maintain for 6 weeks, followed by monthly maintenance until we complete at least 1 year. We chose this pattern because it is the one we use with interstitial cystitis and because it is a midpoint between the ones described in the bibliography (a 72 hours pattern is difficult to implement and a biweekly pattern is too distant).

We have obtained excellent initial results with complete resolution of the hematuria from the first instillation, the patient stays asymptomatic and without recurrence at 6 months of follow-up. The instillations have been well tolerated and no related complications have appeared.

After this period, in the seventh month, the patient spontaneously, without previous urological manipulation, presented a recurrence of the hematuria with secondary anemia that required endoscopic evacuation of clots and a selective embolization of the hypogastric arteries to control it. Due to previous satisfactory response to DMSO, it is decided to return to the instillations, with a good subsequent evolution in the following 4 months.


Conclusions

Secondary bladder amyloidosis is a rare entity manifested mainly as a painless hematuria, sometimes difficult to control. The cystoscopic study shows images that vary from a polypoid mass to nonspecific inflammatory lesions, being the histopathological study essential. It seems that intravesical instillations with dimethyl sulfoxide could be established as a safe option with promising results.


Acknowledgments

We want to thank our colleagues from the Internal Medicine and Pathology departments, who managed the patient during the hospitalization period and processed the samples, helping us to make an accurate diagnosis.


Footnote

Conflicts of Interest: The authors have no conflicts of interest to declare.

Ethical Statement: The authors are accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved. Written informed consent was obtained from the patient for publication of this Case report and any accompanying images.


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Cite this article as: García Rojo E, González Padilla DA, Castelo Loureiro A, Parrilla Rubio L, Tejido Sánchez Á, Guerrero Ramos F. New case of secondary bladder amyloidosis with massive hematuria—role of intravesical instillations with dimethyl sulfoxide. Transl Androl Urol 2019;8(5):548-555. doi: 10.21037/tau.2019.09.22