AB025. The effects and mechanisms of magnetic nanomaterials in prostate cancer diagnosis and therapy
Podium Lecture

AB025. The effects and mechanisms of magnetic nanomaterials in prostate cancer diagnosis and therapy

Li Zhang

Department of Urology, the First Affiliated Hospital of Anhui Medical University, Institute of Urology, Anhui Medical University, Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei 230000, China


Background: Take iron oxides (Fe3O4) nanoclusters for instance, to explore the effects and mechanisms of magnetic nanomaterials in prostate cancer diagnosis and therapy.

Methods: Fe3O4 nanoclusters (Fe3O4 NCs) were synthesized by using hydrothermal method through iron (III) acetylacetonate. The as-prepared Fe3O4 NCs were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), X-ray powder diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR). The T2-weighted image was obtained with a 3.0 T clinical MRI scanner. To establishing PC3-GFP-LC3, a PC3 cell line stably expressing green-fluorescent-protein-tagged microtubule-associated protein 1 light chain 3 (GFP-MAP1LC3), and then evaluate the effect of Fe3O4 NCs upon cell proliferation. To use 4 W/cm2 near infrared laser (NIR, 808 nm), the light-thermal conversion of Fe3O4 NCs was assessed. GFP-LC3 punctate dots were observed by an invert microscope and LC3-I/LC3-II conversion was detected by western blotting. Autophagosome formation was observed by TEM. The tumoricidal effects to PC3 were evaluated by cell proliferation assay in vitro and xenograft volume curve in vivo under NIR in the presence or absence of autophagy inhibitors.

Results: The as-prepared hydrophilic and magnetic Fe3O4 NCs were 100 nm in uniform size. XRD and FTIR analyses showed the NCs possessed the characterized peak and functional motifs, r2 value could reach 143 mM-1S-1, after magnetic targeting, the r2 value could further shorten. Fe3O4 NCs did not affect the cell proliferation at 0–400 µg/mL, indicating the good biocompatibility. Fe3O4 NCs could induce the medium temperature elevated in a time- and dose-dependent manner under 808 nm NIR irradiation. Besides, Fe3O4 NCs could induce complete autophagic flux in PC3 cells, which effect could be inhibited by 3-MA or CQ administration. Under 808 nm NIR, Fe3O4 NCs could elicit 40% cell viability reduction, this reduction could be further enhanced when co-treated with 3-MA or CQ. In vivo study showed that under 808 nm NIR, tumor volume in NS, 3-MA, CQ group increased with prolonged time, while Fe3O4 NCs administration could inhibit the volume increase, when 3-MA or CQ was administrated simultaneously, the tumor volume was sustained with the initial treatments, the body weight of mice in each group did not alter significantly.

Conclusions: Fe3O4 NCs are safe T2-MRI contrast nano-contrast agents, could enhance the sensitivity of prostate cancer diagnosis. The NCs possess outstanding light-thermal conversion capacity, could induce autophagy in PC3 cells. More importantly, Fe3O4 NCs could further enhance the tumoricidal effects under 808 nm NIR when the autophagiy-inducing effects are inhibited in PC3 cells.

Keywords: Magnetic nanomaterials; prostate cancer; diagnosis and therapy


doi: 10.21037/tau.2018.AB025


Cite this article as: Zhang L. The effects and mechanisms of magnetic nanomaterials in prostate cancer diagnosis and therapy. Transl Androl Urol 2018;7(Suppl 5):AB025. doi: 10.21037/tau.2018.AB025