AB246. Effect of hypoxia inducible factor mediated EMT in the mechanism of detrusor instability

Background: To detect the effect of hypoxia inducible factor mediated EMT in the mechanism of detrusor instability.

Methods: A total of 49 patients with detrusor instability (DI) secondary to bladder outlet obstruction (BOO) were randomly chosen according to the urodynamic result as the study group. 36 patients with BOO proved with detrusor instability (DS) by urodynamic as control group. The detrusor specimens were obtained in operation. The expression of E-cadherin, detrusor collagen content and HIF were determined in the detrusor muscle of two groups respectively. Statistical analysis was carried out on the measured results by compute. Changes of E-cadherin, collagen content and HIF were compared.

Results: The expression of E-cadherin was in the DI group was significantly lower than the DS group, collagen and HIF were significantly increased than the DS group (P<0.05).

Conclusions: The histology and molecular biology were changed caused by the BOO, may through mediated the EMT, inhibited the activity of calcium mucin, lead to E-cadherin as the main constituent of intercellular adhesion failure, and then epithelial cells transferred into mesenchymal cells. Therefore we suggest that the interaction between them may represent a novel therapeutic target for the development of novel target based therapies of DI.

Keywords: HIF; bladder outlet obstruction (BOO); detrusor instability; cadherin