AB241. Cancer stem cell-like side population cells in clear cell renal cell carcinoma cell line 769P
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AB241. Cancer stem cell-like side population cells in clear cell renal cell carcinoma cell line 769P

Bin Huang, Dao-Hu Wang, Jun-Xing Chen, Shao-Peng Qiu

The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China


Background: Although cancers are widely considered to be maintained by stem cells, the existence of stem cells in renal cell carcinoma (RCC) has seldom been reported, in part due to the lack of unique surface markers. We here identified cancer stem cell-like cells with side population (SP) phenotype in five human RCC cell lines.

Methods: We here identified cancer stem cell-like cells with side population (SP) phenotype in five human RCC cell lines.

Results: Flow cytometry analysis revealed that 769P, a human clear cell RCC cell line, contained the largest amount of SP cells among five cell lines. These 769P SP cells possessed characteristics of proliferation, self-renewal, and differentiation, as well as strong resistance to chemotherapy and radiotherapy that were possibly related to the ABCB1 transporter. In vivo experiments with serial tumor transplantation in mice also showed that 769P SP cells formed tumors in NOD/SCID mice.

Conclusions: Taken together, these results indicate that 769P SP cells have the properties of cancer stem cells, which may play important roles in tumorigenesis and therapy-resistance of RCC.

Keywords: Cancer stem cell; side population cells; renal cell carcinoma; 769P cell line


doi: 10.21037/tau.2016.s241


Cite this abstract as: Huang B, Wang DH, Chen JX, Qiu SP. Cancer stem cell-like side population cells in clear cell renal cell carcinoma cell line 769P. Transl Androl Urol 2016;5(Suppl 1):AB241. doi: 10.21037/tau.2016.s241

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