AB161. Rottlerin-induced autophagy leads to the apoptosis in bladder cancer cells
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AB161. Rottlerin-induced autophagy leads to the apoptosis in bladder cancer cells

Ping Qi, Zhiping Wang

Urological Surgery Institute of Lanzhou University Second Hospital, Gansu Province Urinary System Disease Research Key Laboratory, Gansu Province Urinary System Disease Clinical Medicine Center, Lanzhou 730000, China


Objective: The aim of this study was to investigate the effects of rottlerin on growth inhibition as well as autophagy in EJ cells in vitro.

Methods: Cell viability, measured by MTT assay, apoptosis of cells was stained by annexin V-FITC/PI staining.

Results: Cell viability, measured by MTT assay, was suppressed in a dose-dependent and time-dependent manner. Moreover, apoptosis of cells was significantly increased with the treatment of rottlerin as indicated by increased annexin V-FITC/PI staining and cell cycle distribution being blocked in G1 phases. Rottlerin treatment also enhanced activation of autophagy with increased expression of LC3-II and appearance of autophagosome.

Conclusions: The increased level of protein LC3-II and autophagosome suggests that autophagy may contribute to apoptosis. In addition, Pro caspase-3, cleaved caspase-3, total PARP, and cleaved-PARP showed no apparent changes in cells treated with rottlerin, which indicates that caspases may not play a key role during the process of apoptosis induced by rottlerin.

Keywords: Autophagy; poptosis; rottlerin; bladder cancer


doi: 10.21037/tau.2016.s161


Cite this abstract as: Qi P, Wang Z. Rottlerin-induced autophagy leads to the apoptosis in bladder cancer cells. Transl Androl Urol 2016;5(Suppl 1):AB161. doi: 10.21037/tau.2016.s161

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